Efficient fluorescence inhibition patterns for RESOLFT microscopy
نویسندگان
چکیده
منابع مشابه
Efficient fluorescence inhibition patterns for RESOLFT microscopy.
By exploiting the saturation of a reversible single photon transition, RESOLFT microscopy is capable of resolving three dimensional structures inside specimen with a resolution that is no longer limited by the wavelength of the light in use. The transition is driven by a spatially varying intensity distribution that features at least one isolated point, line or plane with zero intensity and the...
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Hippocampal brain slices were prepared by dissecting hippocampi from postnatal day P5–P7 wildtype C57BL/6 mice, which were then sectioned in 400 μm thick slices and embedded in a plasma clot on 0.14 mm thick glass coverslips. The slices were maintained in a roller incubator at 35°C in medium containing (in ml): BME 97, HBSS 50, horse serum 50, glucose (5M) 2, glutamine (200mM) 1 — according to ...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2007
ISSN: 1094-4087
DOI: 10.1364/oe.15.003361